Anti-Rabbit IgG H&L/HRP antibody

Quantity size:

100ul

Concentration:

2mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.

Also known as:

Goat Anti-Rabbit IgG H&L /HRP; Immunoglobulin G;

Specificity:

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Goat Polyclonal , affinity purified by Protein G.

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Reacts with: Rabbit, .

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Immunogen: Native Rabbit IgG.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:5000-20000  ELISA=1:5000-20000  IHC-P=1:3000-5000  IHC-F=1:3000-5000  
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.