Recombinant Murine Keratinocyte Growth Factor-2/FGF-10

重组小鼠角质形成细胞生长因子-2/FGF-10

SourceE

scherichia coli.

Molecular Weight

Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

AA Sequence

QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol.EndotoxinLess than 1 EU/µg of rMuKGF-2/FGF-10 as determined by LAL method.